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  • Bank Hinrichsen posted an update 3 years, 2 months ago

    The cDNA samples derived from the petroleumtreated group and control were designated as T and C, respectively.The RPKM value was calculated as follows: RPKM N, and the exon length was used to determine differentially expressed genes.The false discovery rate was set to determine the threshold of P value in multiple tests.The PCR reactions were carried out as follows: C for s; cycles of C for s, C for s, and C for s; and melting curve analysis.The relative fold differences of gene expression were calculated according to the CT method using the S rRNA gene as an internal reference.After trimming off the adaptor sequences and removing lowquality sequences, a total of, and, clean reads with Q values over were obtained for samples T and C, respectively. The N length and mean length of these unigenes were and bp, respectively.The length distribution of assembled sequences were not shorter than bp.HZ may be a special species with a high GC content.Eleven genes were signicantly downregulated values less than. Within the cellular component category, cell and cell part were the most dominant subcategories.A part of genes involved in the same metabolic pathway were differentially affected.Among the inuenced functional categories, the basic structural components, physiological functions, and biological processes were the most dominant, such as cell, cell part, binding, catalytic, cellular process, and metabolic process, as revealed by GO classication. Besides, differential expression analysis revealed that the coding products of genes associated with cell motility were reduced. The reasons why the genes related to cell motility were downregulated, although strain HZ was a motile marine buy Cobimetinib bacterium petroleum treatment might be an environmental stress for strain HZ because petroleum hydrocarbons could be toxic to bacterial cells the environmental stress resulted in downregulation of specic genes, including genes related to cell motility.However, the exact underlying mechanisms remain to be elucidated.Our previous study had shown that strain HZ could partially degrade polycyclic aromatic hydrocarbons. The transcriptional regulators of multiple antibiotic resistance regulator family play an important role in regulation of bacterial responses to antibiotics and catabolism of aromatic compounds. Further experiments are necessary to clarify the drug resistance spectra of strain HZ and to explore why several genes related to drug resistance in this bacterium were upregulated following petroleum treatment.GntR family transcriptional regulators are related to the degradation of aromatic compounds. Besides, genes encoding enzymes indispensable for the oxidization and decarboxylation of pyruvate were downregulated, including genes coding for pyruvate decarboxylase, pyruvate dehydrogenase E component, dihydrolipoyl dehydrogenase, and dihydrolipoamide dehydrogenase pathway were downregulated. S, several steps of the citrate cycle were inhibited.Thus, these results suggest that, without glucose, the glycometabolism pathway might be inactive in the bacterial cells following petroleum treatment.Polymorphum gilvum SLBAT, a hydrocarbondegrading bacterium, was reported to contain several genes for biosurfactant synthesis, such as genes encoding oxoacyl. These enzymes coordinate with each other, step by step, to carry out diverse reactions such as reactions of redox, decarboxylation, deamination, and hydrolysis, completing the degradation processes of substrates. Alkane degradation is initiated by oxygenation under the action of oxygenases or hydroxylases.